Device
Part:BBa_K598013:Design
Designed by: Xiaowei YAN, Tong MU Group: iGEM11_pku_riboclamp (2011-09-27)
pBAD+theoHH+CdIntron+E0040+B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1433
Design Notes
The ligand response is alter from c-di-GMP to theophylline by merging a theophylline hammerhead ribozyme to the intron.
Source
The intron derives from the genome of Cd